Diverse and newly recognized effects associated with siRNA binding site modifications on the Tomato bushy stunt virus P19 silencing suppressor Авторы

Abstract

The Tomato bushy stunt virus (TBSV) encoded P19 forms dimers that bind duplex short interfering RNAs (siRNAs) to suppress RNA silencing. P19 is also involved in multiple hostspecific activities, including the elicitation of symptoms, and in local and/or systemic spread. To study the correlation between those various roles and the siRNA binding by P19, predicted siRNA-interacting sites were modified. Twenty-two mutants were generated and inoculated onto Nicotiana benthamiana plants, to reveal that: i) they were ...